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Loxodes nuclei FACS separation detailed protocol
Oct 18, 2023
Seah, Kwee Boon Brandon; Singh, Aditi; Emmerich, Christiane; Peters, Moritz; Soltys, Volker; Swart, Estienne, 2023, "Loxodes nuclei FACS separation detailed protocol", https://doi.org/10.17617/3.OYUXDS, Edmond, V1
Detailed protocol for isolation of micro- and macronuclei from Loxodes ciliates with fluorescence activated cell sorting (FACS).
Western blots of Loxodes ciliate nuclei for histones and histone modifications
Western blots of Loxodes ciliate nuclei for histones and histone modifications
Oct 18, 2023
Seah, Kwee Boon Brandon; Singh, Aditi; Emmerich, Christiane; Swart, Estienne, 2023, "Western blots of Loxodes ciliate nuclei for histones and histone modifications", https://doi.org/10.17617/3.0DVGMU, Edmond, V1
See README.md for more information
Immunofluorescence of Loxodes ciliate nuclei for histones, histone marks and 6mA
Immunofluorescence of Loxodes ciliate nuclei for histones, histone marks and 6mA
Oct 18, 2023
Seah, Kwee Boon Brandon; Emmerich, Christiane; Singh, Aditi; Swart, Estienne, 2023, "Immunofluorescence of Loxodes ciliate nuclei for histones, histone marks and 6mA", https://doi.org/10.17617/3.VWAUYE, Edmond, V1
Localization of histones, histone modifications, and 6mA base modification to specific nuclei types in Loxodes ciliates by immunofluorescence and imaging of whole cells. This serves to verify results from Western blotting of isolated nuclei populations sorted by FACS. See README....
Soil extract medium for Loxodes culture
May 30, 2022
Seah, Kwee-Boon-Brandon; Emmerich, Christiane; Singh, Aditi; Swart, Estienne, 2022, "Soil extract medium for Loxodes culture", https://doi.org/10.17617/3.4ZQNBK, Edmond, V1
Chemical composition of soil extract medium for cultivation of ciliates, and cell count data for growth rates of ciliates grown in this medium. Refer to README for more information.
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