Genetic and ecological architecture of life cycle transitions in the kelp Laminaria pallida

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Genetic and ecological architecture of life cycle transitions in the kelp Laminaria pallida
Liesner, Daniel, 2026, "Genetic and ecological architecture of life cycle transitions in the kelp Laminaria pallida", https://doi.org/10.17617/3.36JFOY, Edmond, V1
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Description

Description of the data and file structure

Many organisms alternate between distinct life stages and can reproduce either sexually or asexually, yet the mechanisms coordinating these developmental and reproductive transitions remain poorly understood. Here, we investigate the molecular basis of life stage identity and reproductive mode in the kelp Laminaria pallida, a multicellular organism with a complex life cycle. By combining gene expression analyses with developmental experiments and natural population-level approaches, we identify the genetic networks that govern developmental state transitions in both sexual and asexual contexts.

Files and variables

File: Growth.xlsx

Description: Area measurements of Laminaria pallida sporophytes obtained by parthenogenesis and sexual reproduction.

Variables
• strain: single strain or cross identifier
• date: date of the measurement in yyymmdd format
• area_mm2: area of individual sporophytes

File: Exclusion_counts.xlsx

Description: Developmental experiment on Laminaria pallida gametophytes to identify whether mate presence has an influence on parthenogenetic reproduction.

Variables
• replicate: individual ID for each petri dish
• strain: strain or cross identifier
• mate: absence or presence of mate of the opposite sex in the medium
• day: timepoint of the measurement
• fields: fields of view counted
• area_cm2: area conted based on fields of view
• total_gam: absolute number of gametophytes counted
• gam_cm2: density of gametophytes / cm2
• m_veg: number of vegetative male gametophytes
• m_mat: number of reproductive male gametophytes
• m_tra: number of male transition phenotypes
• m_pSP_abs: number of male parthenosporophytes
• f_veg: number of vegetative female gametophytes
• f_mat: number of reproductive female gametophytes
• f_egg: number of female gametophytes with released eggs
• f_pSP_abs: number of female parthenosporophytes
• all_pSP_abs: number of all parthenosporophytes
• eggs_abs: absolute number of released eggs

File: Timecourse_counts.xlsx

Description: Developmental experiment on Laminaria pallida gametophytes to quantify parthenogenetic reproduction.

Variables
• replicate: individual ID for each petri dish
• strain: strain or cross identifier
• setup: single strain or cross of male and female
• day: timepoint of the measurement
• fields: fields of view counted
• area_cm2: area conted based on fields of view
• total_gam: absolute number of gametophytes counted
• gam_cm2: density of gametophytes / cm2
• m_veg: number of vegetative male gametophytes
• m_mat: number of reproductive male gametophytes
• m_tra: number of male transition phenotypes
• f_veg: number of vegetative female gametophytes
• f_mat: number of reproductive female gametophytes
• f_egg: number of female gametophytes with released eggs
• f_sporo: number of female gametophtes with attached sporophytes
• sporo_abs: absolute number of (partheno-)sporophytes

File: Microsat_GenAlEx.xlsx

Description: Microsatellite genotypes of Laminaria pallida sporophytes collected in Paternoster, South Africa (ZAF) and Swakopmund, Namibia (NAM) in GenAlEx format.

Variables
• Individual: individual ID for each sporophyte
• Quadrat: Quadrat within each population where sample was taken
• CN766672_tri: microsatellite marker with two allele values
• Ld_25_penta: microsatellite marker with two allele values
• Ld_67_tri: microsatellite marker with two allele values
• Lo_15_tri: microsatellite marker with two allele values
• Lo_24_tri: microsatellite marker with two allele values
• Lo_26_tetra: microsatellite marker with two allele values
• Lo_27_tetra: microsatellite marker with two allele values
• Lo_28_tetra: microsatellite marker with two allele values
• Lo5-8_tri: microsatellite marker with two allele values

File: S1_raw_images.tif

Description: Annotated raw image file of agarose gel of PCR products with amplified products of sex-specific PCR markers for each Laminaria pallida gametophyte strain and offspring sporophyte lineage used in this study.

Subject Biology
Keyword Laminaria pallida, brown aglae
Related Publication Genetic and ecological architecture of life cycle transitions in a kelp
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1 to 6 of 6 Files
Exclusion_counts.xlsx
MS Excel Spreadsheet - 34.6 KB
Published Oct 2, 2026
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MD5: e965dfeed93cd1e4e4cc9f718cf58e04
Developmental experiment on Laminaria pallida gametophytes to identify whether mate presence has an influence on parthenogenetic reproduction. Variables • replicate: individual ID for each petri dish • strain: strain or cross identifier • mate: absence or presence of mate of the opposite sex in the medium • day: timepoint of the measurement • fields: fields of view counted • area_cm2: area conted based on fields of view • total_gam: absolute number of gametophytes counted • gam_cm2: density of gametophytes / cm2 • m_veg: number of vegetative male gametophytes • m_mat: number of reproductive male gametophytes • m_tra: number of male transition phenotypes • m_pSP_abs: number of male parthenosporophytes • f_veg: number of vegetative female gametophytes • f_mat: number of reproductive female gametophytes • f_egg: number of female gametophytes with released eggs • f_pSP_abs: number of female parthenosporophytes • all_pSP_abs: number of all parthenosporophytes • eggs_abs: absolute number of released eggs
Growth.xlsx
MS Excel Spreadsheet - 10.5 KB
Published Oct 2, 2026
0 Downloads
MD5: 6ccd38411d1c2f0d231a71c0a56c73c8
Area measurements of Laminaria pallida sporophytes obtained by parthenogenesis and sexual reproduction. Variables • strain: single strain or cross identifier • date: date of the measurement in yyymmdd format • area_mm2: area of individual sporophytes
Microsat_GenAlEx.xlsx
MS Excel Spreadsheet - 68.5 KB
Published Oct 2, 2026
0 Downloads
MD5: c959074eb9eaba9b0e0e4ae94b3cf68a
Microsatellite genotypes of Laminaria pallida sporophytes collected in Paternoster, South Africa (ZAF) and Swakopmund, Namibia (NAM) in GenAlEx format. Variables • Individual: individual ID for each sporophyte • Quadrat: Quadrat within each population where sample was taken • CN766672_tri: microsatellite marker with two allele values • Ld_25_penta: microsatellite marker with two allele values • Ld_67_tri: microsatellite marker with two allele values • Lo_15_tri: microsatellite marker with two allele values • Lo_24_tri: microsatellite marker with two allele values • Lo_26_tetra: microsatellite marker with two allele values • Lo_27_tetra: microsatellite marker with two allele values • Lo_28_tetra: microsatellite marker with two allele values • Lo5-8_tri: microsatellite marker with two allele values
README.rtf
Rich Text Format - 11.2 KB
Published Oct 2, 2026
0 Downloads
MD5: 01d30b4463a753278e3bbb8fc661376c
S1_raw_images.tif
S1_raw_images.tif
TIFF Image - 1.7 MB
Published Oct 2, 2026
0 Downloads
MD5: 7dab51577b7ae3814f737c2e1769401b
Annotated raw image file of agarose gel of PCR products with amplified products of sex-specific PCR markers for each Laminaria pallida gametophyte strain and offspring sporophyte lineage used in this study.
Timecourse_counts.xlsx
MS Excel Spreadsheet - 26.0 KB
Published Oct 2, 2026
0 Downloads
MD5: f72f6b1f76678d2e95ec2e84ed329d22
Developmental experiment on Laminaria pallida gametophytes to quantify parthenogenetic reproduction. Variables • replicate: individual ID for each petri dish • strain: strain or cross identifier • setup: single strain or cross of male and female • day: timepoint of the measurement • fields: fields of view counted • area_cm2: area conted based on fields of view • total_gam: absolute number of gametophytes counted • gam_cm2: density of gametophytes / cm2 • m_veg: number of vegetative male gametophytes • m_mat: number of reproductive male gametophytes • m_tra: number of male transition phenotypes • f_veg: number of vegetative female gametophytes • f_mat: number of reproductive female gametophytes • f_egg: number of female gametophytes with released eggs • f_sporo: number of female gametophtes with attached sporophytes • sporo_abs: absolute number of (partheno-)sporophytes
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